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the method comprises introducing into the prokaryotic organism an expressible nucleic acid encoding a putatively essential gene for proliferation or pathogenicity under the control of a promoter and at least one tet operator, and an expression vector comprising a nucleotide sequence encoding a modified tetracycline repressor, wherein said modified tetracycline repressor binds to a tetracycline operator sequence in a prokaryotic organism with a greater affinity in the presence of tetracycline or a tetracycline analog than in the absence of tetracycline or a tetracycline analog. In still further embodiments, the isolated nucleic acid molecules encode a revTetR comprising a sequence of nucleotides including at least one revTetR mutation, and preferably having at least 35%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 98% or 99% nucleotide sequence identity, more preferably at least 90%, 95%, 98% or 99% sequence identity, to any of the nucleotide sequences set forth in SEQ ID NOS. The supposed superiority of bactericidal agents over bacteriostatic agents is of little relevance when treating the vast majority of infections with gram-positive bacteria, buy accutane without a rx particularly in patients with uncomplicated infections and noncompromised immune systems. At times, korean accutane 10mg
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